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> checklist for vibrating microtome leica
checklist for vibrating microtome leica
Pre-Operation Checks
Ensure the microtome is properly plugged in and powered on.
Check that the blade is sharp and in good condition.
Verify that the sample holder is clean and free of debris.
Confirm the water bath is filled with appropriate medium (if applicable).
Inspect the vibration settings and ensure they are properly calibrated.
Sample Preparation
Label samples clearly with relevant information.
Ensure samples are properly embedded and oriented for sectioning.
Check that the samples are at the correct temperature for sectioning.
Setting Up the Microtome
Adjust the microtome settings according to the thickness required for sections.
Set the appropriate vibration frequency based on the sample type.
Secure the sample in the holder ensuring it is stable and aligned.
ing Process
Begin sectioning, maintaining a steady hand and consistent speed.
Monitor the section quality and make adjustments to the settings if necessary.
Collect sections in the water bath or on slides as they are cut.
Post-Operation Procedures
Clean the microtome blade and sample holder after use.
Remove any leftover samples and clean the work area.
Record any observations or issues encountered during sectioning in the logbook.
Maintenance Checks
Inspect the microtome for any signs of wear or damage.
Schedule routine maintenance as per manufacturer recommendations.
Calibrate the equipment periodically to ensure optimal performance.
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